Drug resistance is one of the major challenges in paediatric cancer. Treating paediatric high-risk neuroblastoma, which is the most common extracranial solid tumour in children under one-year-old, is difficult as it frequently develops therapy resistance, leading to treatment failure, tumour relapse, and higher mortality rates. Unfortunately, there are currently limited therapeutic options available for resistant high-risk neuroblastoma. Therefore, there is a need to develop more targeted treatment strategies to overcome therapy resistance. The process of therapy resistance is not entirely understood, but it is believed to be driven by multiple molecular mechanisms, including genetic alterations and epigenetic changes.
We are seeking a highly motivated and talented pre-doctoral candidate to develop a thesis project in the field of molecular biology of neuroblastoma. The candidate will be part of a research project that has received competitive funding from the Instituto de Salud Carlos III. The project aims to investigate the molecular mechanisms underlying therapy resistance to develop more effective treatment strategies and improve outcomes for neuroblastoma patients. The project includes using a range of molecular biology techniques and muti-omic approaches, including cell cultures and cell lines, animal models, and tissue samples from neuroblastoma patients.
The successful candidate will be responsible for tasks intrinsic to a Ph.D. thesis, including literature review and critical analysis, hypothesis formulation, experimental design and implementation, data analysis, interpretation, and manuscript preparation for publication in peer-reviewed journals.
The experiments will encompass functional in vitro studies in cultured cells, molecular techniques such as real-time qPCR, immunoblot, and immunofluorescence, as well as in vivo studies using rodent models to evaluate the effectiveness of new treatments.
Additionally, the candidate will use patient tissue samples to validate the research findings.
Throughout the thesis development, the candidate will also employ various multi-omic approaches to comprehensively characterize molecular alterations in drug-resistant patients. Furthermore, the candidate will collaborate closely with an interdisciplinary team of clinicians and researchers.
Other tasks:
• Biological sample extraction, collection and processing.
• Participation in the animal experimentation procedures.
• Contribution to the publication of research results in national and international journals.
• Presentation of research results at national and international conferences.
• Data analysis and visualization.
• Comply with the 4Rs (reduction, reuse, recycling and recovery) and ethics involving patient samples and animals in research.
